glial cell line  (R&D Systems)

Journal: The FASEB Journal

Article Title: CSPG4 Mediates Inflammatory, Cell Death, and Senescence Responses in Enteric Glia Exposed to Clostridioides difficile Toxins

doi: 10.1096/fj.202600333R

Figure Lengend Snippet: Human, mouse, and rat enteric glia express all C. difficile toxin receptors. Gene expression of C. difficile toxin receptors: Cspg4 , Tfpi, Lrp1, Pvrl3, Fzd1, Fzd3, Fzd7 , and Ldlr in human and mouse enteric glia, obtained through the Single‐Cell Portal (“the human and mouse enteric nervous system at single cell resolution”) . Gene expression of these receptors in rat enteric glia (PK060399egfr/ATCC) cells was measured by qPCR.

Article Snippet: Immortalized rat enteric glial cell line PK060399egfr (ATCC CRL‐2690, VA, USA) was cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco) and supplemented with 10% bovine serum albumin (BSA), 1% antibiotics (100 μg/mL penicillin and 100 μg/mL streptomycin, Gibco), and 1 mM sodium pyruvate (Gibco) at 37°C in a humidified incubator under 5% CO 2 for no more than 24 passages.

Techniques: Gene Expression, Single Cell

Journal: Journal of Advanced Research

Article Title: A cocktail hydrogel promoting the functional interneurons regeneration of human neural progenitor cells for brain injury therapy

doi: 10.1016/j.jare.2025.05.063

Figure Lengend Snippet: The in vitro release behavior of neural inducers and biocompatibility of BGA@GelMA hydrogel. (A to C) The relative release profile of BDNF, GDNF, and cAMP of the BGA@GelMA hydrogel measured with ELISA kit, n = 3. (D) Schematic representation of 3D culture system via the BGA@GelMA and the Matrigel hydrogel. (E) Flow cytometry was used to detect APC of cell apoptosis. (F) Statistical diagram of apoptotic cell distribution, n = 3, *** p = 0.0005. (G) Quantitative analysis of live cells and dead cells per field in the live/dead assay, n = 3. (H) Representative live/dead images of hNPCs co-cultured with the BGA@GelMA hydrogels and the Matrigel hydrogels after 14 days.

Article Snippet: The release kinetics of neural inducers in BGA@GelMA hydrogel were assessed using the human BDNF valukine enzyme-linked immunosorbent assay (ELISA) kit (VAL136, R&D Systems), GDNF ELISA kit (CSB-E04565h, Cusabio) and cAMP ELISA kit (CSB-E04488h, Cusabio), respectively.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Live Dead Assay, Cell Culture

Journal: Journal of Advanced Research

Article Title: A cocktail hydrogel promoting the functional interneurons regeneration of human neural progenitor cells for brain injury therapy

doi: 10.1016/j.jare.2025.05.063

Figure Lengend Snippet: The in vitro release behavior of neural inducers and biocompatibility of BGA@GelMA hydrogel. (A to C) The relative release profile of BDNF, GDNF, and cAMP of the BGA@GelMA hydrogel measured with ELISA kit, n = 3. (D) Schematic representation of 3D culture system via the BGA@GelMA and the Matrigel hydrogel. (E) Flow cytometry was used to detect APC of cell apoptosis. (F) Statistical diagram of apoptotic cell distribution, n = 3, *** p = 0.0005. (G) Quantitative analysis of live cells and dead cells per field in the live/dead assay, n = 3. (H) Representative live/dead images of hNPCs co-cultured with the BGA@GelMA hydrogels and the Matrigel hydrogels after 14 days.

Article Snippet: The release kinetics of neural inducers in BGA@GelMA hydrogel were assessed using the human BDNF valukine enzyme-linked immunosorbent assay (ELISA) kit (VAL136, R&D Systems), GDNF ELISA kit (CSB-E04565h, Cusabio) and cAMP ELISA kit (CSB-E04488h, Cusabio), respectively.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Live Dead Assay, Cell Culture